Time course and regional distribution of cortical changes during acute alcohol ingestion

Behavioral effects of alcohol are known to be greater when the blood alcohol is rising, known as the Mellanby effect; however, research investigating the cortical changes during this period is scarce. The objective of this study was to investigate the effects of consumption of alcohol on cortical activity measured by the electroencephalogram (EEG) during the absorption or rising phase of alcohol EEG signals were recorded using the entire 10/20 montage system. The experimental design consisted of a repeated measures randomized crossover design in which subjects acted as their own control. This involved recording two EEG baseline measures, each of which was followed by a placebo or alcohol condition, delivered over two days for ten subjects. All subjects had a 50% chance of receiving the alcohol first. All subjects were shown to have mean peak blood alcohol concentration (BAC) levels of around .03%. No significant differences were found between the two baselines. Significant increases in EEG magnitude occurred in the theta (4-7.75 Hz), alpha 1 (8-9.75 Hz), and beta 1 (13.25-19.75 Hz) spectrum in the frontal EEG regions, and alpha 1 (8-9.75 Hz) in the central and posterior regions. No significant changes were found in the theta (4-7.75 Hz) or beta (13.5-30 Hz) spectrums in the central and posterior regions. There were also no significant results for alpha 2 (10-13 Hz) in any of the regions. These results suggest that rapid cortical changes occur within the first 35 min after alcohol consumption

Precursor proteins in transit through mitochondrial contact sites interact with hsp70 in the matrix

We previously reported that hsp70 in the mitochondrial matrix (mt-hsp70 = Ssc1p) is required for import of precursor proteins destined for the matrix or intermembrane space. Here we show that mt-hsp70 is also needed for the import of mitochondrial inner membrane proteins. In particular, the precursor of ADP/ATP carrier that is known not to interact with hsp60 on its assembly pathway requires functional mt-hsp70 for import, suggesting a general role of mt-hsp70 in membrane translocation of precursors. Moreover, a precursor arrested in contact sites was specifically co-precipitated with antibodies directed against mt-hsp70. We conclude that mt-hsp70 is directly involved in the translocation of many, if not all, precursor proteins that are transported across the inner membrane

Late Miocene-Quaternary fault evolution and interaction in the southern California Inner Continental Borderland

Changing conditions along plate boundaries are thought to result in the reactivation of preexisting structures. The offshore southern California Borderland has undergone dramatic adjustments as conditions changed from subduction tectonics to transform tectonics, including major Miocene oblique extension, followed by transpressional fault reactivation. However, consensus is still lacking about stratigraphic age models, fault geometry, and slip history for the near-offshore area between southern Los Angeles and San Diego (California, USA). We interpret an extensive data set of seismic reflection, bathymetric, and stratigraphic data from that area to determine the three-dimensional geometry and kinematic evolution of the faults and folds and document how preexisting structures have changed their activity and type of slip through time. The resulting structural representation reveals a moderately landward-dipping San Mateo–Carlsbad fault that converges downward with the steeper, right-lateral Newport-Inglewood fault, forming a fault wedge affected by Quaternary contractional folding. This fault wedge deformed in transtension during late Miocene through Pliocene time. Subsequently, the San Mateo–Carlsbad fault underwent 0.6–1.0 km displacement, spatially varying between reverse right lateral and transtensional right lateral. In contrast, shallow parts of the previously identified gently dipping Oceanside detachment and the faults above it appear to have been inactive since the early Pliocene. These observations, together with new and revised geometric representations of additional steeper faults, and the evidence for a pervasive strike-slip component on these nearshore faults, suggest a need to revise the earthquake hazard estimates for the coastal region

The dietary impact of the Norman Conquest: A multiproxy archaeological investigation of Oxford, UK

Archaeology has yet to capitalise on the opportunities offered by bioarchaeological approaches to examine the impact of the 11th-century AD Norman Conquest of England. This study utilises an integrated multiproxy analytical approach to identify and explain changes and continuities in diet and foodways between the 10th and 13th centuries in the city of Oxford, UK. The integration of organic residue analysis of ceramics, carbon (δ13C) and nitrogen (δ15N) isotope analysis of human and animal bones, incremental analysis of δ13C and δ15N from human tooth dentine and palaeopathological analysis of human skeletal remains has revealed a broad pattern of increasing intensification and marketisation across various areas of economic practice, with a much lesser and more short-term impact of the Conquest on everyday lifestyles than is suggested by documentary sources. Nonetheless, isotope data indicate short-term periods of instability, particularly food insecurity, did impact individuals. Evidence of preferences for certain foodstuffs and cooking techniques documented among the elite classes were also observed among lower-status townspeople, suggesting that Anglo-Norman fashions could be adopted across the social spectrum. This study demonstrates the potential for future archaeological research to generate more nuanced understanding of the cultural impact of the Norman Conquest of England, while showcasing a method which can be used to elucidate the undocumented, everyday implications of other large-scale political events on non-elites

Elak jadi korban industri 4.0

Supervisors’ ratings of psychology trainees’ competence in field settings are a critical component of training assessment. There has been little systematic research regarding the validity of these assessments, but the available evidence suggests we have a problem! Supervisors’ judgments may be affected by systemic biases that pose a serious threat to assessment credibility. The current study is part of a research collaboration among six universities that endeavors to develop and evaluate a new method—the use of vignettes—against outcomes derived from a conventional rating scale. Individual vignettes were designed and subjected to a rigorous process of peer-review and revisions, before final vignettes were assigned calibration scores by a group of experts. A catalogue of vignettes (n = 41) that represent various domains of competence across several developmental stages was compiled. University and field supervisors used the conventional rating scale and the vignette-matching procedure (VMP) to evaluate competencies at end-placement. Data from a pilot (n = 20) and a follow-up study (n = 57) suggest that compared with a conventional rating scale, the VMP reduced leniency and halo biases. The VMP has the potential to improve outcomes of competency assessments in field placements and merits further research and development

Dominant components of the Thoroughbred metabolome characterised by 1H‐NMR spectroscopy: a metabolite atlas of common biofluids

Summary Reasons for performing study: Metabonomics is emerging as a powerful tool for disease screening and investigating mammalian metabolism. This study aims to create a metabolic framework by producing a preliminary reference guide for the normal equine metabolic milieu. Objectives: To metabolically profile plasma, urine and faecal water from healthy racehorses using high resolution 1H-NMR spectroscopy and to provide a list of dominant metabolites present in each biofluid for the benefit of future research in this area. Study design: This study was performed using seven Thoroughbreds in race training at a single time-point. Urine and faecal samples were collected non-invasively and plasma was obtained from samples taken for routine clinical chemistry purposes. Methods: Biofluids were analysed using 1H-NMR spectroscopy. Metabolite assignment was achieved via a range of 1D and 2D experiments. Results: A total of 102 metabolites were assigned across the three biological matrices. A core metabonome of 14 metabolites was ubiquitous across all biofluids. All biological matrices provided a unique window on different aspects of systematic metabolism. Urine was the most populated metabolite matrix with 65 identified metabolites, 39 of which were unique to this biological compartment. A number of these were related to gut microbial host co-metabolism. Faecal samples were the most metabolically variable between animals; acetate was responsible for the majority (28%) of this variation. Short chain fatty acids were the predominant features identified within this biofluid by 1H-NMR spectroscopy. Conclusions: Metabonomics provides a platform for investigating complex and dynamic interactions between the host and its consortium of gut microbes and has the potential to uncover markers for health and disease in a variety of biofluids. Inherent variation in faecal extracts along with the relative abundance of microbial-mammalian metabolites in urine and invasive nature of plasma sampling, infers that urine is the most appropriate biofluid for the purposes of metabonomic analysis

The influence of microRNAs and poly(A) tail length on endogenous mRNA–protein complexes

Background: All mRNAs are bound in vivo by proteins to form mRNA-protein complexes (mRNPs), but changes in the composition of mRNPs during posttranscriptional regulation remain largely unexplored. Here, we have analyzed, on a transcriptome-wide scale, how microRNA-mediated repression modulates the associations of the core mRNP components eIF4E, eIF4G, and PABP and of the decay factor DDX6 in human cells. Results: Despite the transient nature of repressed intermediates, we detect significant changes in mRNP composition, marked by dissociation of eIF4G and PABP, and by recruitment of DDX6. Furthermore, although poly(A)-tail length has been considered critical in post-transcriptional regulation, differences in steady-state tail length explain little of the variation in either PABP association or mRNP organization more generally. Instead, relative occupancy of core components correlates best with gene expression. Conclusions: These results indicate that posttranscriptional regulatory factors, such as microRNAs, influence the associations of PABP and other core factors, and do so without substantially affecting steady-state tail length.National Institutes of Health (U.S.) (Grant K99GM102319)National Institutes of Health (U.S.) (Grant T32GM007753)National Institutes of Health (U.S.) (Grant R01GM067031)National Institutes of Health (U.S.) (Grant R35GM118135)Natural Sciences and Engineering Research Council of Canada (Discovery Grant

EXPORTS North Atlantic eddy tracking

The EXPORTS North Atlantic field campaign (EXPORTS-NA) of May 2021 used a diverse array of ship-based and autonomous platforms to measure and quantify processes leading to carbon export in the open ocean. The success of this field program relied heavily on the ability to make measurements following a Lagrangian trajectory within a coherent, retentive eddy (Sections 1, 2). Identifying an eddy that would remain coherent and retentive over the course of a monthlong deployment was a significant challenge that the EXPORTS team faced. This report details the processes and procedures used by the primarily shore-based eddy tracking team to locate, track, and sample with autonomous assets such an eddy before and during EXPORTS-NA.This field deployment was funded by the NASA Ocean Biology and Biogeochemistry program and the National Science Foundation Biological and Chemical Oceanography programs. Initial gliders deployments were performed by the RRS Discovery and the authors thank the Porcupine Abyssal Plain – Sustained Observatory of the Natural Environment Research Council (NERC, UK), which is principally funded through the Climate Linked Atlantic Sector Science (CLASS) project supported by NERC National Capability funding (NE/R015953/1) and by IFADO (Innovation in the Framework of the Atlantic Deep Ocean) EAPA_165/2016. Technical assistance with glider deployment was provided by Marine Autonomous Robotic Systems (NOC). The authors thank Inia Soto Ramos for assistance in publishing this manuscript through the NASA Technical Memorandum series. This is PMEL contribution number 5372